Annotation process
From ReactomeWiki
Complete_tubulin_folding pathway:
Post-chaperonin folding subpathway:
Tubulin Folding pathway outline:
Pathway I. Prefoldin mediated transfer of newly translated tubulin to Chaperonin Reaction 1. Association of prefoldin with newly translated tubulin Reaction 2. Transfer of tubulin from prefoldin to Chaperonin:ADP
Pathway II. Formation of tubulin folding intermediates by Chaperonin Reaction 1. Exchange of ADP for ATP in Chaperonin Reaction 2. Hydrolysis of ATP in the CCT:ATP:Tubulin complex leading to the Reaction 3. Release of tubulin folding intermediate (or unfolded tubulin ) which is Reaction 4. Recaptured by CCT:ADP (goto III)
Pathway III. Formation of the alpha-beta tubulin dimer Reaction 5. Capture of tubulin -alpha or beta folding intermediates by cofactors A-E (reversible rxn) Reaction 6. Association of (Alpha:E) and (beta:D) with CoC Reaction 7. Alpha:beta dimer formation: alpha:E+beta:D+CoC GTP->GDP --> release of the alpha:beta heterodimer
Pathway 1: Prefoldin mediated transfer of newly translated tubulin to Chaperonin Reaction 1: alpha/beta tubulin (defined set) + PFD → alpha/beta tubulin:PDF complex
Reaction 2: alpha/beta tubulin:PDF complex + CCT:ADP complex -> alpha/beta tubulin:PDF:CCT:ADP complex
Reaction 3.Dissociation of PFD from tubulin (no ATP/GTP hydrolysis) alpha/beta tubulin:PDF:CCT:ADP complex-> alpha/beta tubulin:CCT:ADP +PDF
Pathway 2: Formation of tubulin folding intermediates by Chaperonin Reaction 4.ADP is exchanged for ATP in the (ADP:CCT):(GTP:Tubulin) complex (ADP:CCT):(GTP:Tubulin) ->(ATP:CCT):(GTP:Tubulin)
Reaction 5. Conformational change in CCP and Tubulin GTP:Tubulin:CCP:ADP --> GTP:*Tubulin(folding intermediate):CCP*:ATP (how many molecules of ADP->ATP)
Reaction 6. GTP:Tubulin*(Quasi-native folding intermediate):CCP*:ATP-> GTP:*Tubulin(quasi-native folding intermediate) + CCP*:ADP +PPi
Pathway 3: Formation of the alpha-beta tubulin dimer Reaction 7. *Beta-tubulin:GTP + Cofactor A → *Beta-tubulin:GTP: Cofactor A(reversible)
Reaction 8. *Beta-tubulin(:GTP +Cofactor D → *Beta-tubulin:GTP: Cofactor D (reversible)
Reaction 9 *Beta-tubulin:GTP: Cofactor A+ Cofactor D → *Beta-tubulin:GTP: Cofactor D + Cofactor A (reversible)
.Reaction 10 *alpha-tubulin:GTP + Cofactor B -> *alpha-tubulin:GTP:Cofactor B(reversible)
Reaction 11. *alpha-tubulin:GTP + Cofactor E -> *alpha-tubulin:GTP:Cofactor E(reversible)
Reaction 12. *alpha-tubulin:GTP:Cofactor B + Cofactor E-> *alpha-tubulin:GTP:Cofactor E + Cofactor B (reversible)
Reaction 13. Beta-tubulin:GTP:Cofactor D+ alpha-tubulin:GTP:Cofactor E -> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E
Reaction 14. Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E + Cofactor C-> Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E : Cofactor C
Reaction 15 .Beta-tubulin:GTP:Cofactor D:alpha-tubulin:GTP:Cofactor E : Cofactor C-> GDP:Beta-tubulin :alpha-tubulin:GTP heterodimer +Cofactor E+ Cofactor D+ Cofactor C+ PPi